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preparation kit  (Illumina Inc)


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    Structured Review

    Illumina Inc preparation kit
    Preparation Kit, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 11832 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xt+kit/Nextera+XT+DNA+Library+Preparation+Kit/pmc12848573-228-17-20
    Average 99 stars, based on 11832 article reviews
    preparation kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: The microbiota and T cells non-genetically modulate inherited phenotypes transgenerationally
    Article Snippet: RNA was size selected using RNA-Clean XP beads (Beckman Coulter A63987) and full-length polyadenylated RNA was reverse transcribed using Superscript II. .. Resulting cDNA was amplified using 10 cycles and the amplified product was used to construct a pool of uniquely indexed samples using the Nextera XT kit (Illumina FC-131-1096). .. Finally, pooled libraries were sequenced by Illumina NextSeq1000 (paired end).

    Construct:

    Article Title: The microbiota and T cells non-genetically modulate inherited phenotypes transgenerationally
    Article Snippet: RNA was size selected using RNA-Clean XP beads (Beckman Coulter A63987) and full-length polyadenylated RNA was reverse transcribed using Superscript II. .. Resulting cDNA was amplified using 10 cycles and the amplified product was used to construct a pool of uniquely indexed samples using the Nextera XT kit (Illumina FC-131-1096). .. Finally, pooled libraries were sequenced by Illumina NextSeq1000 (paired end).

    Article Title: Genome sequence of a vesicular stomatitis Indiana virus isolate collected in 1988 from a naturally infected bovine in Mexico
    Article Snippet: First-strand cDNA synthesis was performed using SuperScript III RT (Invitrogen) and tagged random and oligo dT primers, second-strand synthesis was performed with Sequenase (Affymetrix) and the dsDNA was amplified with TaKaRa Taq Version 2.0 (TakaraBio) ( ). .. A sequencing library was constructed with the Nextera XT kit (Illumina) and sequenced with a paired-end 500-cycle MiSeq Reagent Kit v2 on an Illumina MiSeq platform. .. Bioinformatic analyses were performed in CLC Genomics Workbench (Qiagen) v21.0.4 using default parameters.

    Sequencing:

    Article Title: Genome sequence of a vesicular stomatitis Indiana virus isolate collected in 1988 from a naturally infected bovine in Mexico
    Article Snippet: First-strand cDNA synthesis was performed using SuperScript III RT (Invitrogen) and tagged random and oligo dT primers, second-strand synthesis was performed with Sequenase (Affymetrix) and the dsDNA was amplified with TaKaRa Taq Version 2.0 (TakaraBio) ( ). .. A sequencing library was constructed with the Nextera XT kit (Illumina) and sequenced with a paired-end 500-cycle MiSeq Reagent Kit v2 on an Illumina MiSeq platform. .. Bioinformatic analyses were performed in CLC Genomics Workbench (Qiagen) v21.0.4 using default parameters.

    Article Title: Targeted Rapamycin Delivery via Magnetic Nanoparticles to Address Stenosis in a 3D Bioprinted in Vitro Model of Pulmonary Veins.
    Article Snippet: The cDNA from samples was generated using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio, USA). .. The final sequencing library was made using the Nextera XT kit (Illumina, USA). ..

    Article Title: Evidence of reassortment of avian influenza A (H2) viruses in Brazilian shorebirds
    Article Snippet: .. The libraries for sequencing were prepared with a Nextera XT Kit (Illumina, San Diego, USA) using the PCR product as input, according to manufacturer’s instructions. .. The resulting libraries were quantified, mixed at equimolar amounts and submitted to 150 bp paired-end reads using an Illumina NextSeq 550 sequencing system (Illumina).

    Polymerase Chain Reaction:

    Article Title: Identification and Localization of Cell Types in the Mouse Olfactory Bulb using Slide-SeqV2
    Article Snippet: .. Note 8 : The library preparation described is performed according to manufacturer’s directions for the Nextera XT kit (Illumina, FC-131–1096), modifying the starting input of material to 600pg of cDNA and using a specific P5-Truseq PCR hybrid oligo in place of the Nextera XT i5 adapter. ..

    Article Title: Evidence of reassortment of avian influenza A (H2) viruses in Brazilian shorebirds
    Article Snippet: .. The libraries for sequencing were prepared with a Nextera XT Kit (Illumina, San Diego, USA) using the PCR product as input, according to manufacturer’s instructions. .. The resulting libraries were quantified, mixed at equimolar amounts and submitted to 150 bp paired-end reads using an Illumina NextSeq 550 sequencing system (Illumina).



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